Corinne RAVELET

Parcours et intérêts

1999 : Doctorat spécialité Méthodes de recherche pour l'Environnement et la Santé - UJF, Grenoble. « Biodégradation du pyrène par des micromycètes dans un sédiment de rivière ».
2000 : ATER, UJF, chimie analytique.
2002 : PostDoctorat, Cémagref, Lyon.
2003 : Maitre de Conférence des Universités

Projets

• Sélection d'oligonucléotides fonctionnels. La méthode SELEX, dont l'unité est l'un des rares laboratoires français à disposer, permet la sélection d'oligonucléotides fonctionnels, en particulier pour des cibles présentant un intérêt clinique ou toxicologique. L'objectif principal de ce projet est de sélectionner des aptamères dédiés aux biomarqueurs d'oligoéléments et des petites molécules, jusqu'aux peptides ou des protéines, tout en utilisant une alternative au SELEX : la méthode CE-SELEX.

• Mise au point de dispositifs d'analyse innovants. Ce projet comprend deux volets : d'une part, il s'agit de développer des tests de diagnostic faciles à utiliser, rapides, peu coûteux et/ou portables à l'aide d'aptamères, afin de facilité les soins aux patients ou de détecter les contaminants environnementaux ; d'autre part, l'objectif est d'établir de nouvelles stratégies de signalement pour le développement de dispositifs à haut débit et/ou haute sensibilité.

Publications

• Article • Stability of fecal calprotectin extracts using the Diasorin® kit [Full paper ]

  B. Billet, L. Choisnard, P. Faure, D. Guicherd, E. Cussigh, E. Peyrin, C. Ravelet, B. Chovelon

  Clin. Chem. Lab. Med. 2024, 62, e13–e15

• Article • Stability of catecholamines in whole blood: Influence of time between collection and centrifugation [Full paper ]

  Q. Despinasse, L. Choisnard, P. Faure, D. Guicherd, E. Peyrin, C. Ravelet, B. Chovelon

  Clin. Chem. Lab. Med. 2021, 59, e83 85

• Article • Iodinated Organic Molecule as Tag for Inductively Coupled Plasma-Mass Spectrometry Aptamer Assays [Full paper ]

  B. Billet, B. Chovelon, E. M. McConnell, D. André, L. Puillet-Anselme, E. Fiore, P. Faure, C. Ravelet, M. C. DeRosa, E. Peyrin

  Talanta, 2024, 267, 125107

• Article • Non-Competitive Determination of Small Analytes by Sandwich-type Lateral Flow Assay based on Aptamer Kissing Complex [Full paper ]

  B. Chovelon, V. Ranganathan, S. Srinivasan, E. McConnell, P. Faure, E. Fiore, C. Ravelet, E. Peyrin, M. DeRosa

  Anal. Chem. 2024, 96, 18, 6875-6880

• Article • Nile blue as reporter dye in salt aggregation based-colorimetric aptasensors for peptide, small molecule and metal ion detection [Full paper ]

  B. Chovelon, E. Peyrin, M. Ragot, N. Salem, T. Giang Nguyen, B. Auvray, M. Henry, M-A Petrillo, E. Fiore, Q. Bessy, P. Faure, C. Ravelet

  Anal. Chim. Acta, 2023, 1243, 340840

• Article • Detection of Small Molecules by Fluorescence Intensity using Single Dye Labeled Aptamers and Quencher Transition Metal Ions [Full paper ]

  B. Billet, B. Chovelon, E. Fiore, P. Faure, C. Ravelet, E. Peyrin

  Biosens. Bioelectron. 2022, 205, 114091

• Article • ATP7B-Deficient Hepatocytes Reveal the Importance of Protein Misfolding Induced at Low Copper Concentration [Full paper ]

  P. Charbonnier, B. Chovelon, C. Ravelet, T. D. Ngo, M. Chevallet and A. Deniaud

  Cells, 2022, 11, 3400

• Article • Aptamer Switches Regulated by Post-Transition/Transition Metal Ions [Full paper ]

  B. Billet, B. Chovelon, E. Fiore, F. Oukacine, M-A Petrillo, P. Faure, C. Ravelet, E. Peyrin

  Angew. Chem. Int. Ed. 2021, 60, 12346–12350

• Article • Anti-pesticide DNA aptamers fail to recognize their targets with asserted micromolar dissociation constants [Full paper ]

  L. Zara, S. Achilli, B. Chovelon, E. Fiore, J.J Toulmé, E. Peyrin, C. Ravelet,

  Anal. Chim. Acta 2021, 1159, 338382

• Article • Melting Curve Analysis of Aptachains: Adenosine Detection with Internal Calibration [Full paper ]

  C. Lu, C. Saint-Pierre, D. Gasparutto, Y. Roupioz, C. Ravelet, E. Peyrin, A. Buhot

  Biosensors 2021, 11, 112

• Article • Peptide-based CE-SELEX enables convenient isolation of aptamers specifically recognizing CD20-expressing cells 

  J. Cossu, C. Ravelet, V. Martel-Frachet, E. Peyrin, D. Boturyn

  Bioorg. Med. Chem. 2024, doi.org/10.1016/j.bmc.2024.117831

• Article • Non-SELEX Isolation of DNA Aptamers for the Homogeneous-Phase Fluorescence Anisotropy Sensing of Tau Proteins [Full paper ]

  S. Lisi, E. Fiore, S. Scarano, E. Pascale, Y. Boehmann, F. Ducongé, S. Chierici, M. Minnuni, E. Peyrin, C. Ravelet

  Anal. Chim. Acta 2018, 1038, 173-181

• Article • Efficient functional neutralization of lethal peptide toxins in vivo by oligonucleotides [Full paper ]

  T. Abd El Aziz, C. Ravelet, J. Molgo, E. Fiore, S. Pale, M. Amar, S. Al Khoury, J. Dejeu, M. Fadl, M. Ronjat, G. Sotoing Taiwe, D. Servent, E. Peyrin, M. De Waard

  Sci. Rep., 2017, 7, 7202

• Article • Chiral resolution capabilities of DNA oligonucleotides [Full paper ]

  L. Tohala, F. Oukacine, C. Ravelet, E. Peyrin

  Anal. Chem. 2015, 87, 5491-5495.

• Article • An improved design of the kissing complex-based aptasensor for the detection of adenosine [Full paper ]

  E Goux, S Lisi, C Ravelet, G Durand, E Fiore, E Dausse, JJ Toulmé, E Peyrin

  Anal. Bioanal. Chem. 2015, 407, 6515-6524.

• Article • Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing  [Full paper ]

  E. Fiore, E. Dausse, H. Dubouchaud, E. Peyrin, C. Ravelet

  Front. Chem. 2015, 3, 49

• Article • Riboswitches Based on Kissing Complexes for the Detection of Small Ligands. [Full paper ]

  G. Durand, S. Lisi, C. Ravelet, E. Dausse, E. Peyrin, JJ. Toulmé.

  Angew. Chem. Int. Ed. 2014,53,6942-6945.

• Article • Enantiomeric sensing and separation by nucleic acids [Full paper ]

  F. Oukacine, C. Ravelet, E. Peyrin

  Trends Anal. Chem. 2020, 122, 115733

• Article • Surface functionalization of gold nanoclusters with arginine: a trade-off between microtumor uptake and radiotherapy enhancement. 

  M. Broekgaarden, A. L. Bulin, E. Porret, B. Musnier, B. Chovelon, C. Ravelet, L. Sancey, H. Elleaume, P. Hainaut, J. L. Coll, X. Le Guével

  Nanoscale 2020, 12, 6959-6963

• Article • Influence of delayed separation of plasma from whole blood & centrifugation protocol on Zn plasma concentration [Full paper ]

  M. Leger, Q. Despinasse, P. Faure, J. Arnaud, C. Ravelet, B. Chovelon

  Clin Chem Lab Med e279-e281

• Article • Mirror-Image Aptamer Kissing Complex for Arginine-Vasopressin Sensing [Full paper ]

  B. Chovelon, E. Fiore, P. Faure, E. Peyrin, C. Ravelet

  Anal. Chim. Acta, 2018, 1001, 143-150

• Article • Panoply of Fluorescence Polarization/Anisotropy Signaling Mechanisms for Functional Nucleic Acid-Based Sensing Platforms [Full paper ]

  S. Perrier, V. Guieu, B. Chovelon, C. Ravelet, E. Peyrin

  Anal. Chem. 2018, 90, 4236–4248.

• Article • Toward sensitive immuno-based detection of Tau protein by surface plasmon resonance coupled to carbon nanostructures as signal amplifiers [Full paper ]

  S. Lisi, S. Scarano, S. Fedeli, E. Pascale, S. Cicchi, C. Ravelet, E. Peyrin, M. Minunni

  Biosens. Bioelectron. 2017, 93, 289-292.

• Article • A Lifetime-Sensitive Fluorescence Anisotropy Probe for DNA-based Bioassays: the Case of SYBR Green [Full paper ]

  B. Chovelon, E. Fiore, P. Faure, E. Peyrin, C. Ravelet

  Biosens. Bioelectron. 2017, 90, 140-145.

• Article • Colorimetric nanosensor based on a selective target-responsive aptamer kissing complex [Full paper ]

  E. Goux, E. Dausse, V. Guieu, L. Azéma, G. Durand, M. Henry, L. Choisnard, J-J. Toulmé, C. Ravelet, E. Peyrin

  Nanoscale, 2017, 9, 4048 - 4052.

• Article • Sequence Requirements of Oligonucleotide Chiral Selectors for the Capillary Electrophoresis Resolution of Low-Affinity DNA Binders [Full paper ]

  L . Tohala, F. Oukacine, C. Ravelet, E. Peyrin

  Electrophoresis, 2017, 38, 1383-1390

• Article • Fluorescence Anisotropy-Based Structure-Switching Aptamer Assay Using a Peptide Nucleic Acid (PNA) Probe [Full paper ]

  E. Goux, Q. Lespinasse, V. Guieu, S. Perrier, C. Ravelet, E. Fiore, E. Peyrin

  Methods, 2016, 97, 69–74

• Article • ELAKCA: Enzyme-Linked Aptamer Kissing Complex Assay as a Small Molecule Sensing Platform [Full paper ]

  B. Chovelon, G. Durand, E. Dausse, JJ. Toulme, P. Faure , E. Peyrin, C. Ravelet

  Anal. Chem., 2016, 88, 2570-2575

• Article • A combinatorial approach to the repertoire of RNA kissing motifs; toward multiplex detection by switching hairpin aptamers [Full paper ]

  G. Durand, E. Dausse, E. Goux, E. Fiore, E. Peyrin, C. Ravelet, J-J. Toulmé

  Nucleic Acids Res. 2016, 44, 4450-4459.

• Article • Detecting Alzheimer's Disease biomarkers: from antibodies to new bio-mimetic receptors and their application to established and emerging bioanalytical platforms - A critical review [Full paper ]

  S. Scarano, S. Lisi, C. Ravelet, E. Peyrin, M. Minunni

  Anal. Chim. Acta, 2016, 940, 21-37

• Article • Multi-analytical study of the binding between a small chiral molecule and a DNA aptamer: evidences for asymmetric steric effect upon 3’ vs. 5’-end sequence modification [Full paper ]

  L. Challier, R. Miranda-Castro, B. Barbe, C. Fave, B. Limoges, E. Peyrin, C. Ravelet, E. Fiore, P. Labbé, L. Coche-Guérente, E. Ennifar, G. Bec, P. Dumas, F. Mavré, V. Noël

  Anal. Chem. 2016, 88, 11963–11971

• Article • Catalytic DNA-Based Fluorescence Polarization Chiral Sensing Platform for L-Histidine Detection at Trace Level [Full paper ]

  N. Braga Amaral, S. Zuliani, V. Guieu, C. Ravelet, S. Perrier, E. Peyrin.

  Anal. Bioanal. Chem. 2014, 406, 1173-1179.

• Article • Capillary Gel Electrophoresis-Coupled Aptamer Enzymatic Cleavage Protection Strategy for the Simultaneous Detection of Multiple Small Analytes [Full paper ]

  S. Perrier, Z. Zhu, E. Fiore, C. Ravelet, V. Guieu, E. Peyrin.

  Anal. Chem. 2014, 86, 4233-4240.

• Patent • Kits-of-parts comprising nucleic acids able to form a kissing complex and their uses thereof 

  G. Durand, C. Ravelet, E. Dausse, E. Peyrin, JJ. Toulmé

  2013 • Inserm, UJF, CNRS

• Article • Single-Stranded DNA Binding Protein-Assisted Fluorescence Polarization Aptamer Assay for Detection of Small Molecules [Full paper ]

  Z. Zhu, C. Ravelet, S. Perrier, V. Guieu, E. Fiore, E. Peyrin.

  Anal. Chem. 2012, 84, 7203-7211.

• Article • Aptamer Enzymatic Cleavage Protection Assay for the Gold Nanoparticle-Based Colorimetric Sensing of Small Molecules. [Full paper ]

  V. Guieu, C. Ravelet, S. Perrier, Z. Zhu, S. Cayez, E. Peyrin.

  Anal. Chim. Acta 2011, 706, 349-353.

• Article • Optimization of the Structure-Switching Aptamer-Based Fluorescence Polarization Assay for the Sensitive Tyrosinamide Sensing [Full paper ]

  Z. Zhu, T. Schmidt, M. Mahrous, V. Guieu, S. Perrier, C. Ravelet, E. Peyrin.

  Anal. Chim. Acta 2011, 707, 191-196.

• Article • Fluorescence polarization biosensor based on aptamer enzymatic cleavage protection strategy [Full paper ]

  A. Kidd, V. Guieu, S. Perrier, C. Ravelet, E. Peyrin

  Anal. Bioanal. Chem. 2011, 401, 3229-3234.

• Article • Utilisation des aptamères pour la détection de petites molécules par polarisation de fluorescence 

  V. Guieu, C. Ravelet, S. Perrier, E. Peyrin

  Spectra analyse 2011, 282, 24-31

• Article • Investigation of low energy proton effects on aptamer performances for astrobiological applications 

  M. Bacqué,A. Le Postollec,C. Ravelet,E. Peyrin, G. Coussot,I. Desvignes,S. Incerti, P. Moretto,M. Dobrijevic,O. Vandenabeele-Trambouze.

  Astrobiology 2011, 11, 207-211.

• Article • Rationally Designed Aptamer-Based Fluorescence Polarization Sensor Dedicated to the Small Target Analysis 

  S. Perrier, C. Ravelet, V. Guieu, J. Fize, B. Roy, C. Perigaud, E. Peyrin.

  Biosens. Bioelectron. 2010, 25, 1652-1657.

• Article • Multiplexed detection of small analytes by structure-switching aptamer-based capillary electrophoresis 

  Z. Zhu, C. Ravelet, S. Perrier, V. Guieu, B. Roy, C. Perigaud, E. Peyrin.

  Anal. Chem. 2010, 82, 4613-4620.

• Book • Aptamers in bioanalysis 

  Aptamers for separation of enantiomers

  C. Ravelet, E. Peyrin

  2009 • Wiley

• Article • Chiral ligand-exchange chromatography of amino acids using porous graphitic carbon coated with a dinaphthyl derivative of neamine. 

  M. Zaher, C. Ravelet, I. Baussanne, A. Ravel, C. Grosset, J.-L Decout, E. Peyrin.

  Anal. Bioanal. Chem. 2009, 393, 655-660.

• Article • Aptamer-Modified Micellar Electrokinetic Chromatography for the Enantioseparation of Nucleotides. 

  J. Ruta, S. Perrier, C. Ravelet, B. Roy, C. Perigaud, E. Peyrin.

  Anal. Chem. 2009, 81, 1169-1176.

• Article • Enantioseparation by micellar electrokinetic chromatography using a ligand exchange-based chiral pseudostationary phase 

  M. Zaher, C. Ravelet, C. Vanhaverbeke, I. Baussanne, S. Perrier, J. Fize, J.-L. Decout, E. Peyrin

  Electrophoresis, 2009, 30, 2869-2873

• Book • Functional Nucleic Acids for Analytical Applications 

  Aptamers in affinity separations

  C. Ravelet, E. Peyrin

  2009 • Springer

• Article • Noncompetitive fluorescence polarization aptamer-based assay for small molecule detection 

  J. Ruta, S. Perrier, C. Ravelet, J. Fize, E. Peyrin.

  Anal. Chem. 2009, 81, 7468-7473.

• Article • Covalently bonded DNA aptamer chiral stationary phase for the chromatographic resolution of adenosine. 

  J. Ruta, C. Ravelet, J. Désiré, J.-L. Décout, E. Peyrin.

  Anal. Bioanal. Chem. 2008, 390, 1051-1057.

• Article • Copper(II) complexes of lipophilic aminoglycoside derivatives for the amino acid enantiomeric separation by ligand-exchange liquid chromatography. [Full paper ]

  M. Zaher, I. Baussanne, C. Ravelet, S. Halder, M. Haroun, J. Fize, J.-L. Decout, E. Peyrin.

  J Chromatogr A. 2008, 1185, 291-295.

• Article • Competitive affinity capillary electrophoresis assay based on a hybrid pre-incubation/on-capillary mixing format using an enantioselective aptamer as affinity ligand. 

  J. Ruta, C. Ravelet, I. Baussanne, J. Fize, J.-L. Decout, E. Peyrin.

  J. Sep.Sci. 2008, 31, 2239-2243

• Article • Chiral resolution of histidine using an anti-D-histidine L-RNA aptamer microbore column 

  J. Ruta, C. Grosset, C. Ravelet, J. Fize, A. Villet, A. Ravel, E. Peyrin

  J. Chromatogr. B 2007, 845, 186-190.

• Patent • Chiral selectors for separating enantiomers of a compound. 

  E. Peyrin, J.-L. Décout, C. Ravelet, I. Baussanne.

  2007 • Université de Grenoble I/CNRS

• Article • Aptamer-based enantioselective competitive binding assay for the trace enantiomer detection. 

  J. Ruta, C. Ravelet, I. Baussanne, J.-L. Decout, E. Peyrin.

  Anal. Chem. 2007, 79, 4716-4719.

• Article • Reversal of the enantiomeric elution order of some aromatic amino acids using reversed-phase chromatographic supports coated with the teicoplanin chiral selector 

  M. Haroun, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Peyrin

  Talanta 2006, 68, 1032-1036.

• Article • Enantiomeric separation using a L-RNA aptamer as chiral additive in partial-filling capillary electrophoresis. 

  J. Ruta, C. Ravelet, C. Grosset, J. Fize, A. Ravel, A. Villet, E. Peyrin.

  Anal. Chem. 2006, 78, 3032-3039.

• Article • Recent developments in the HPLC enantiomeric separation using chiral selectors identified by a combinatorial strategy 

  C. Ravelet, E. Peyrin

  J. Sep. Sci. 2006, 29, 1322-1331.

• Article • Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers 

  C. Ravelet, C. Grosset, E. Peyrin

  J. Chromatogr. A 2006, 1117, 1-10.

• Article • Chiral stationary phase based on a biostable L-RNA aptamer 

  A. Brumbt,C. Ravelet,C. Grosset,A. Ravel,A. Villet, E. Peyrin

  Anal. Chem. 2005, 77, 1993-1998.

• Article • Thermodynamic origin of the chiral recognition of tryptophan on teicoplanin and teicoplanin aglycone stationary phases 

  M. Haroun,C. Ravelet,A. Ravel,C. Grosset,A. Villet, E. Peyrin

  J. Sep. Sci. 2005, 28, 409-420.

• Article • Semi-permeable membrane device efficiency for sampling free soluble fraction of polycyclic aromatic hydrocarbons 

  C. Miege,C. Ravelet,J. P. Croue,J. Garric

  Anal. Chim. Acta 2005, 536, 259-266.

• Article • A L-RNA aptamer chiral stationary phase for the resolution of target and related compounds 

  C. Ravelet, R. Boulkedid, A. Ravel, C. Grosset, A. Villet, J. Fize, E. Peyrin

  J. Chromatogr. A 2005, 1076, 62-70.

• Article • How precisely do Semi-Permeable Membrane Devices (SPMD) measure the bioavailability of Polycyclic Aromatic Hydrocarbons to Daphnia magna ? 

  C. Gourlay, C. Miege, A. Noir, C. Ravelet, J. Garric, J. M. Mouchel

  Chemosphere 2005, 61, 1734-1739.

• Article • Streptavidin chiral stationary phase for the separation of adenosine enantiomers 

  C. Ravelet, M. Michaud, A. Ravel, C. Grosset, A. Villet, E. Peyrin

  J. Chromatogr. A 2004, 1036, 155-160.

• Article • Immobilized DNA aptamers as target-specific chiral stationary phases for resolution of nucleoside and amino acid derivative enantiomers 

  M. Michaud, E. Jourdan, C. Ravelet, A. Villet, A. Ravel, C. Grosset, E. Peyrin

  Anal. Chem. 2004, 76, 1015-1020

• Article • A framework based on the extended Wyman concept for analyzing the salt effects on the solute retention in high performance affinity chromatography 

  I. Slama, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Nicolle, E. Peyrin

  Anal. Chem. 2002,74, 282-287

• Article • Stoichiometry and formation constants of six PAHs with gamma-cyclodextrin determined by high performance liquid chromatography using a cyano stationary phase 

  C. Ravelet, A. Ravel, C. Grosset, A. Villet, A. Geze, D. Wouessidjewe, E. Peyrin

  J. Liq. Chromatogr. Relat. Technol. 2002, 25, 421-432

• Article • A displacement study on vancomycin based-stationary phase using N-acetyl-D-alanine as a competing agent 

  I. Slama, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Nicolle, E. Peyrin

  J. Chromatogr. Sci. 2002, 40, 83-86

• Article • Chromatographic determination of the association constants between nimesulide and native and modified beta-cyclodextrins 

  C. Ravelet, A. Geze, A. Villet, C. Grosset, A. Ravel D. Wouessidjewe, E. Peyrin

  J. Pharm. Biomed. Anal. 2002, 29, 425-429