Corinne RAVELET

Maître de conférences au DPM depuis le 01-09-2003

Bureau : E203

rf.sepla-elbonerg-vinu@televaR.enniroC

Tel : 0476635306

Fax : 0476635298

 Equipe « NOVA »

Parcours et intérêts

1999 : Doctorat spécialité Méthodes de recherche pour l'Environnement et la Santé - UJF, Grenoble. « Biodégradation du pyrène par des micromycètes dans un sédiment de rivière ».
2000 : ATER, UJF, chimie analytique.
2002 : PostDoctorat, Cémagref, Lyon.
2003 : Maitre de Conférence des Universités

Projets

  • Sélection d'oligonucléotides fonctionnels. La méthode SELEX, dont l'unité est l'un des rares laboratoires français à disposer, permet la sélection d'oligonucléotides fonctionnels, en particulier pour des cibles présentant un intérêt clinique ou toxicologique. L'objectif principal de ce projet est de sélectionner des aptamères dédiés aux biomarqueurs d'oligoéléments et des petites molécules, jusqu'aux peptides ou des protéines, tout en utilisant une alternative au SELEX : la méthode CE-SELEX.

  • Mise au point de dispositifs d'analyse innovants. Ce projet comprend deux volets : d'une part, il s'agit de développer des tests de diagnostic faciles à utiliser, rapides, peu coûteux et/ou portables à l'aide d'aptamères, afin de facilité les soins aux patients ou de détecter les contaminants environnementaux ; d'autre part, l'objectif est d'établir de nouvelles stratégies de signalement pour le développement de dispositifs à haut débit et/ou haute sensibilité.

Publications

  • Article • Anti-pesticide DNA aptamers fail to recognize their targets with asserted micromolar dissociation constants 

    L. Zara, S. Achilli, B. Chovelon, E. Fiore, J.J Toulmé, E. Peyrin, C. Ravelet,

    Analytica Chimica Acta, 2021, accepted

  • Article • Aptamer efficacies for in vitro and in vivo modulation of alphaC-conotoxin PrXA pharmacology 

    G. Taiwe, J. Montnach, S. Nicolas, S. De Waard, E. Fiore, E. Peyrin, T. El Aziz, M. Amar, J. Molgo, M. Ronjat, D. Servent, C. Ravelet, M. De Waard

    Molecules accepted

  • Article • Non-SELEX Isolation of DNA Aptamers for the Homogeneous-Phase Fluorescence Anisotropy Sensing of Tau Proteins 

    S. Lisi, E. Fiore, S. Scarano, E. Pascale, Y. Boehmann, F. Ducongé, S. Chierici, M. Minnuni, E. Peyrin, C. Ravelet

    Anal. Chim. Acta 2018, 1038, 173-181

  • Article • Efficient functional neutralization of lethal peptide toxins in vivo by oligonucleotides 

    T. Abd El Aziz, C. Ravelet, J. Molgo, E. Fiore, S. Pale, M. Amar, S. Al Khoury, J. Dejeu, M. Fadl, M. Ronjat, G. Sotoing Taiwe, D. Servent, E. Peyrin, M. De Waard

    Sci. Rep., 2017, 7, 7202

  • Article • Aptamer Switches Regulated by Post-Transition/Transition Metal Ions [Full paper ]

    B. Billet, B. Chovelon, E. Fiore, F. Oukacine, M-A Petrillo, P. Faure, C. Ravelet, E. Peyrin

    Angew. Chem. Int. Ed. 2021, 60, 12346–12350

  • Article • Melting curve analysis of Aptachains: Adenosine detection with internal calibration 

    C. Lu, C. Saint-Pierre, D. Gasparutto, Y. Roupioz, C. Ravelet, E. Peyrin, A. Buhot

    Biosensors, 2021, 11, 112

  • Article • Kissing Interactions for the Design of a Multicolour Fluorescence Anisotropy Chiral Aptasensor 

    B. Chovelon, E. Fiore, P. Faure, E. Peyrin, C. Ravelet

    Talanta, accepted

  • Article • Mirror-Image Aptamer Kissing Complex for Arginine-Vasopressin Sensing 

    B. Chovelon, E. Fiore, P. Faure, E. Peyrin, C. Ravelet

    Anal. Chim. Acta, 2018, 1001, 143-150

  • Article • Panoply of Fluorescence Polarization/Anisotropy Signaling Mechanisms for Functional Nucleic Acid-Based Sensing Platforms 

    S. Perrier, V. Guieu, B. Chovelon, C. Ravelet, E. Peyrin

    Anal. Chem. 2018, 90, 4236–4248.

  • Article • Toward sensitive immuno-based detection of Tau protein by surface plasmon resonance coupled to carbon nanostructures as signal amplifiers 

    S. Lisi, S. Scarano, S. Fedeli, E. Pascale, S. Cicchi, C. Ravelet, E. Peyrin, M. Minunni

    Biosens. Bioelectron. 2017, 93, 289-292.

  • Article • A Lifetime-Sensitive Fluorescence Anisotropy Probe for DNA-based Bioassays: the Case of SYBR Green 

    B. Chovelon, E. Fiore, P. Faure, E. Peyrin, C. Ravelet

    Biosens. Bioelectron. 2017, 90, 140-145.

  • Article • Colorimetric nanosensor based on a selective target-responsive aptamer kissing complex 

    E. Goux, E. Dausse, V. Guieu, L. Azéma, G. Durand, M. Henry, L. Choisnard, J-J. Toulmé, C. Ravelet, E. Peyrin

    Nanoscale, 2017, 9, 4048 - 4052.

  • Article • Sequence Requirements of Oligonucleotide Chiral Selectors for the Capillary Electrophoresis Resolution of Low-Affinity DNA Binders 

    L . Tohala, F. Oukacine, C. Ravelet, E. Peyrin

    Electrophoresis, 2017, 38, 1383-1390

  • Article • Fluorescence Anisotropy-Based Structure-Switching Aptamer Assay Using a Peptide Nucleic Acid (PNA) Probe 

    E. Goux, Q. Lespinasse, V. Guieu, S. Perrier, C. Ravelet, E. Fiore, E. Peyrin

    Methods, 2016, 97, 69–74

  • Article • ELAKCA: Enzyme-Linked Aptamer Kissing Complex Assay as a Small Molecule Sensing Platform 

    B. Chovelon, G. Durand, E. Dausse, JJ. Toulme, P. Faure , E. Peyrin, C. Ravelet

    Anal. Chem., 2016, 88, 2570-2575

  • Article • A combinatorial approach to the repertoire of RNA kissing motifs; toward multiplex detection by switching hairpin aptamers 

    G. Durand, E. Dausse, E. Goux, E. Fiore, E. Peyrin, C. Ravelet, J-J. Toulmé

    Nucleic Acids Res. 2016, 44, 4450-4459.

  • Article • Detecting Alzheimer's Disease biomarkers: from antibodies to new bio-mimetic receptors and their application to established and emerging bioanalytical platforms - A critical review 

    S. Scarano, S. Lisi, C. Ravelet, E. Peyrin, M. Minunni

    Anal. Chim. Acta, 2016, 940, 21-37

  • Article • Multi-analytical study of the binding between a small chiral molecule and a DNA aptamer: evidences for asymmetric steric effect upon 3’ vs. 5’-end sequence modification 

    L. Challier, R. Miranda-Castro, B. Barbe, C. Fave, B. Limoges, E. Peyrin, C. Ravelet, E. Fiore, P. Labbé, L. Coche-Guérente, E. Ennifar, G. Bec, P. Dumas, F. Mavré, V. Noël

    Anal. Chem. 2016, 88, 11963–11971

  • Article • Chiral resolution capabilities of DNA oligonucleotides [Full paper ]

    L. Tohala, F. Oukacine, C. Ravelet, E. Peyrin

    Anal. Chem. 2015, 87, 5491-5495.

  • Article • An improved design of the kissing complex-based aptasensor for the detection of adenosine [Full paper ]

    E Goux, S Lisi, C Ravelet, G Durand, E Fiore, E Dausse, JJ Toulmé, E Peyrin

    Anal. Bioanal. Chem. 2015, 407, 6515-6524.

  • Article • Ultrafast Capillary Electrophoresis Isolation of DNA Aptamer for the PCR Amplification-Based Small Analyte Sensing  [Full paper ]

    E. Fiore, E. Dausse, H. Dubouchaud, E. Peyrin, C. Ravelet

    Front. Chem. 2015, 3, 49

  • Article • Riboswitches Based on Kissing Complexes for the Detection of Small Ligands. [Full paper ]

    G. Durand, S. Lisi, C. Ravelet, E. Dausse, E. Peyrin, JJ. Toulmé.

    Angew. Chem. Int. Ed. 2014,53,6942-6945.

  • Article • Copper(II) complexes of lipophilic aminoglycoside derivatives for the amino acid enantiomeric separation by ligand-exchange liquid chromatography. 

    M. Zaher, I. Baussanne, C. Ravelet, S. Halder, M. Haroun, J. Fize, J.-L. Decout, E. Peyrin.

    J Chromatogr A. 2008, 1185, 291-295.

  • Article • Stability of catecholamines in whole blood: Influence of time between collection and centrifugation [Full paper ]

    Q. Despinasse, L. Choisnard, P. Faure, D. Guicherd, E. Peyrin, C. Ravelet, B. Chovelon

    Clinical Chemistry and Laboratory Medicine, 2021, 59, E83-E85

  • Article • Enantiomeric sensing and separation by nucleic acids [Full paper ]

    F. Oukacine, C. Ravelet, E. Peyrin

    Trend. Anal. Chem. 2020, 122, 115733

  • Article • Surface functionalization of gold nanoclusters with arginine: a trade-off between microtumor uptake and radiotherapy enhancement. 

    M. Broekgaarden, A. L. Bulin, E. Porret, B. Musnier, B. Chovelon, C. Ravelet, L. Sancey, H. Elleaume, P. Hainaut, J. L. Coll, X. Le Guével

    Nanoscale 2020, 12, 6959-6963

  • Article • Influence of delayed separation of plasma from whole blood & centrifugation protocol on Zn plasma concentration 

    Mandy Leger, Quentin Despinasse, Patrice Faure, Josiane Arnaud, Corinne Ravelet, Benoit Chovelon

    Clinical Chemistry and Laboratory Medicine, in press

  • Article • Catalytic DNA-Based Fluorescence Polarization Chiral Sensing Platform for L-Histidine Detection at Trace Level 

    N. Braga Amaral, S. Zuliani, V. Guieu, C. Ravelet, S. Perrier, E. Peyrin.

    Anal. Bioanal. Chem. 2014, 406, 1173-1179.

  • Article • Capillary Gel Electrophoresis-Coupled Aptamer Enzymatic Cleavage Protection Strategy for the Simultaneous Detection of Multiple Small Analytes 

    S. Perrier, Z. Zhu, E. Fiore, C. Ravelet, V. Guieu, E. Peyrin.

    Anal. Chem. 2014, 86, 4233-4240.

  • Patent • Kits-of-parts comprising nucleic acids able to form a kissing complex and their uses thereof 

    G. Durand, C. Ravelet, E. Dausse, E. Peyrin, JJ. Toulmé

    2013 • Inserm, UJF, CNRS

  • Article • Single-Stranded DNA Binding Protein-Assisted Fluorescence Polarization Aptamer Assay for Detection of Small Molecules [Full paper ]

    Z. Zhu, C. Ravelet, S. Perrier, V. Guieu, E. Fiore, E. Peyrin.

    Anal. Chem. 2012, 84, 7203-7211.

  • Article • Aptamer Enzymatic Cleavage Protection Assay for the Gold Nanoparticle-Based Colorimetric Sensing of Small Molecules. 

    V. Guieu, C. Ravelet, S. Perrier, Z. Zhu, S. Cayez, E. Peyrin.

    Anal. Chim. Acta 2011, 706, 349-353.

  • Article • Optimization of the Structure-Switching Aptamer-Based Fluorescence Polarization Assay for the Sensitive Tyrosinamide Sensing 

    Z. Zhu, T. Schmidt, M. Mahrous, V. Guieu, S. Perrier, C. Ravelet, E. Peyrin.

    Anal. Chim. Acta 2011, 707, 191-196.

  • Article • Fluorescence polarization biosensor based on aptamer enzymatic cleavage protection strategy 

    A. Kidd, V. Guieu, S. Perrier, C. Ravelet, E. Peyrin

    Anal. Bioanal. Chem. 2011, 401, 3229-3234.

  • Article • Utilisation des aptamères pour la détection de petites molécules par polarisation de fluorescence 

    V. Guieu, C. Ravelet, S. Perrier, E. Peyrin

    Spectra analyse 2011, 282, 24-31

  • Article • Investigation of low energy proton effects on aptamer performances for astrobiological applications 

    M. Bacqué,A. Le Postollec,C. Ravelet,E. Peyrin, G. Coussot,I. Desvignes,S. Incerti, P. Moretto,M. Dobrijevic,O. Vandenabeele-Trambouze.

    Astrobiology 2011, 11, 207-211.

  • Article • Rationally Designed Aptamer-Based Fluorescence Polarization Sensor Dedicated to the Small Target Analysis 

    S. Perrier, C. Ravelet, V. Guieu, J. Fize, B. Roy, C. Perigaud, E. Peyrin.

    Biosens. Bioelectron. 2010, 25, 1652-1657.

  • Article • Multiplexed detection of small analytes by structure-switching aptamer-based capillary electrophoresis 

    Z. Zhu, C. Ravelet, S. Perrier, V. Guieu, B. Roy, C. Perigaud, E. Peyrin.

    Anal. Chem. 2010, 82, 4613-4620.

  • Book • Aptamers in bioanalysis 

    Aptamers for separation of enantiomers

    C. Ravelet, E. Peyrin

    2009 • Wiley

  • Article • Chiral ligand-exchange chromatography of amino acids using porous graphitic carbon coated with a dinaphthyl derivative of neamine. 

    M. Zaher, C. Ravelet, I. Baussanne, A. Ravel, C. Grosset, J.-L Decout, E. Peyrin.

    Anal. Bioanal. Chem. 2009, 393, 655-660.

  • Article • Aptamer-Modified Micellar Electrokinetic Chromatography for the Enantioseparation of Nucleotides. 

    J. Ruta, S. Perrier, C. Ravelet, B. Roy, C. Perigaud, E. Peyrin.

    Anal. Chem. 2009, 81, 1169-1176.

  • Article • Enantioseparation by micellar electrokinetic chromatography using a ligand exchange-based chiral pseudostationary phase 

    M. Zaher, C. Ravelet, C. Vanhaverbeke, I. Baussanne, S. Perrier, J. Fize, J.-L. Decout, E. Peyrin

    Electrophoresis, 2009, 30, 2869-2873

  • Book • Functional Nucleic Acids for Analytical Applications 

    Aptamers in affinity separations

    C. Ravelet, E. Peyrin

    2009 • Springer

  • Article • Noncompetitive fluorescence polarization aptamer-based assay for small molecule detection 

    J. Ruta, S. Perrier, C. Ravelet, J. Fize, E. Peyrin.

    Anal. Chem. 2009, 81, 7468-7473.

  • Article • Covalently bonded DNA aptamer chiral stationary phase for the chromatographic resolution of adenosine. 

    J. Ruta, C. Ravelet, J. Désiré, J.-L. Décout, E. Peyrin.

    Anal. Bioanal. Chem. 2008, 390, 1051-1057.

  • Article • Competitive affinity capillary electrophoresis assay based on a hybrid pre-incubation/on-capillary mixing format using an enantioselective aptamer as affinity ligand. 

    J. Ruta, C. Ravelet, I. Baussanne, J. Fize, J.-L. Decout, E. Peyrin.

    J. Sep.Sci. 2008, 31, 2239-2243

  • Article • Chiral resolution of histidine using an anti-D-histidine L-RNA aptamer microbore column 

    J. Ruta, C. Grosset, C. Ravelet, J. Fize, A. Villet, A. Ravel, E. Peyrin

    J. Chromatogr. B 2007, 845, 186-190.

  • Patent • Chiral selectors for separating enantiomers of a compound. 

    E. Peyrin, J.-L. Décout, C. Ravelet, I. Baussanne.

    2007 • Université de Grenoble I/CNRS

  • Article • Aptamer-based enantioselective competitive binding assay for the trace enantiomer detection. 

    J. Ruta, C. Ravelet, I. Baussanne, J.-L. Decout, E. Peyrin.

    Anal. Chem. 2007, 79, 4716-4719.

  • Article • Reversal of the enantiomeric elution order of some aromatic amino acids using reversed-phase chromatographic supports coated with the teicoplanin chiral selector 

    M. Haroun, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Peyrin

    Talanta 2006, 68, 1032-1036.

  • Article • Enantiomeric separation using a L-RNA aptamer as chiral additive in partial-filling capillary electrophoresis. 

    J. Ruta, C. Ravelet, C. Grosset, J. Fize, A. Ravel, A. Villet, E. Peyrin.

    Anal. Chem. 2006, 78, 3032-3039.

  • Article • Recent developments in the HPLC enantiomeric separation using chiral selectors identified by a combinatorial strategy 

    C. Ravelet, E. Peyrin

    J. Sep. Sci. 2006, 29, 1322-1331.

  • Article • Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers 

    C. Ravelet, C. Grosset, E. Peyrin

    J. Chromatogr. A 2006, 1117, 1-10.

  • Article • Chiral stationary phase based on a biostable L-RNA aptamer 

    A. Brumbt,C. Ravelet,C. Grosset,A. Ravel,A. Villet, E. Peyrin

    Anal. Chem. 2005, 77, 1993-1998.

  • Article • Thermodynamic origin of the chiral recognition of tryptophan on teicoplanin and teicoplanin aglycone stationary phases 

    M. Haroun,C. Ravelet,A. Ravel,C. Grosset,A. Villet, E. Peyrin

    J. Sep. Sci. 2005, 28, 409-420.

  • Article • Semi-permeable membrane device efficiency for sampling free soluble fraction of polycyclic aromatic hydrocarbons 

    C. Miege,C. Ravelet,J. P. Croue,J. Garric

    Anal. Chim. Acta 2005, 536, 259-266.

  • Article • A L-RNA aptamer chiral stationary phase for the resolution of target and related compounds 

    C. Ravelet, R. Boulkedid, A. Ravel, C. Grosset, A. Villet, J. Fize, E. Peyrin

    J. Chromatogr. A 2005, 1076, 62-70.

  • Article • How precisely do Semi-Permeable Membrane Devices (SPMD) measure the bioavailability of Polycyclic Aromatic Hydrocarbons to Daphnia magna ? 

    C. Gourlay, C. Miege, A. Noir, C. Ravelet, J. Garric, J. M. Mouchel

    Chemosphere 2005, 61, 1734-1739.

  • Article • Streptavidin chiral stationary phase for the separation of adenosine enantiomers 

    C. Ravelet, M. Michaud, A. Ravel, C. Grosset, A. Villet, E. Peyrin

    J. Chromatogr. A 2004, 1036, 155-160.

  • Article • Immobilized DNA aptamers as target-specific chiral stationary phases for resolution of nucleoside and amino acid derivative enantiomers 

    M. Michaud, E. Jourdan, C. Ravelet, A. Villet, A. Ravel, C. Grosset, E. Peyrin

    Anal. Chem. 2004, 76, 1015-1020

  • Article • A framework based on the extended Wyman concept for analyzing the salt effects on the solute retention in high performance affinity chromatography 

    I. Slama, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Nicolle, E. Peyrin

    Anal. Chem. 2002,74, 282-287

  • Article • Stoichiometry and formation constants of six PAHs with gamma-cyclodextrin determined by high performance liquid chromatography using a cyano stationary phase 

    C. Ravelet, A. Ravel, C. Grosset, A. Villet, A. Geze, D. Wouessidjewe, E. Peyrin

    J. Liq. Chromatogr. Relat. Technol. 2002, 25, 421-432

  • Article • A displacement study on vancomycin based-stationary phase using N-acetyl-D-alanine as a competing agent 

    I. Slama, C. Ravelet, C. Grosset, A. Ravel, A. Villet, E. Nicolle, E. Peyrin

    J. Chromatogr. Sci. 2002, 40, 83-86

  • Article • Chromatographic determination of the association constants between nimesulide and native and modified beta-cyclodextrins 

    C. Ravelet, A. Geze, A. Villet, C. Grosset, A. Ravel D. Wouessidjewe, E. Peyrin

    J. Pharm. Biomed. Anal. 2002, 29, 425-429