Modulateurs et correcteurs de la protéine F508-del CFTR pour la thérapie de la mucoviscidose  
Targeting different binding sites in the CFTR structures allows to synergistically potentiate channel activity  
[Full paper ]
Lionel Froux ; Ahmad Elbahnsi ; Benjamin Boucherle ; Arnaud Billet ; Nesrine Baatallah ; Brice Hoffmann ; Julien Alliot ; Renaud Zelli ; Wael Zeinyeh ; Romain Haudecoeur ; Antoine Fortuné ; ... ; Jean-Paul Mornon ; Isabelle Callebaut ; Jean-Luc Décout
European Journal of Medicinal Chemistry (2020) 112116
Recent evidence shows that combination of correctors and potentiators, such as the drug ivacaftor (VX-770), can significantly restore the functional expression of mutated Cystic Fibrosis Transmembrane conductance Regulator (CFTR), an anion channel which is mutated in cystic fibrosis (CF). The success of these combinatorial therapies highlights the necessity of identifying a broad panel of specific binding mode modulators, occupying several distinct binding sites at structural level. Here, we identified two small molecules, SBC040 and SBC219, which are two efficient cAMP-independent potentiators, acting at low concentration of forskolin with EC50 close to 1 μM and in a synergic way with the drug VX-770 on several CFTR mutants of classes II and III. Molecular dynamics simulations suggested potential SBC binding sites at the vicinity of ATP-binding sites, distinct from those currently proposed for VX-770, outlining SBC molecules as members of a new family of potentiators.